The J.N. "Ding" Darling National Wildlife Refuge is located on the subtropical barrier island of Sanibel in the Gulf of Mexico. The refuge is part of the largest undeveloped mangrove ecosystem in the United States. It is world famous for its spectacular wading bird populations.

The refuge is located approximately 15 miles southwest of Fort Myers on Sanibel Island. Once on the island, turn right onto Periwinkle Drive. Proceed 3 miles to Palm Ridge Road (marked Captiva and Refuge) and bear right. Go through stop sign and continue 2 miles to refuge entrance on right.